These protocols are currently used in the lab and freely downloadable as PDF files. In any case, they represent any advertisement for any of the company cited. Some of the "kit" protocols have been "optimized".
There is a full version of these protocols compiled in a single downloadable file.
- Nucleic Acid Purification
- Nucleic Acid Analysis
- Molecular cloning
- Microbiology
Preparation of Bacterial Genomic DNA (CETAB version)
Preparation of Fly Genomic DNA (CETAB version)
Quick Fly Genomic DNA prep (BDGP protocol)
Single fly DNA prep for PCR
Plasmid Miniprep Kit Protocol (Alkalin Lysis)
Spin Column Plasmid Miniprep Kit Protocol (Qiagen)
QIAprep Spin Midi/Maxiprep Kit Protocol (Qiagen)
QIAFilter Plasmid
HiSpeed Plasmid
Extraction of Drosophila total RNA
RNeasy MiniPrep Protocol (Qiagen)
RNeasy Cleanup Protocol (Qiagen)
DNA purification on sepharose columns (home-made columns)
QIAquick Gel Extraction Kit Protocol (Qiagen)
QIAquick PCR Purification Kit Protocol (Qiagen)
Southern Blot
Northern Blot
Radioactive Probe labeling
Blot hybridization
Basic PCR Protocols (BioLine)
Expand high Fidelity PCR System (Roche)
Expand Long Template PCR System (Roche)
Reverse transcription
Quantitative PCR (Light Cycler)
Analysis of PCR product by direct restriction
DNA sequencing
Protocol for direct sequencing on genomic DNA (Bacterial DNA)
Colony Blot
Restriction enzyme buffers (Fermentas enzymes)
Restriction enzyme buffers (specific buffers; Fermentas buffers)
Enzyme activity close to DNA extremities (fermentas charts)
Ligation
Ligation of double-stranded oligonucleotides
Universal T/A cloning T-vector preparation
Recombineering
Antibiotics and additives
Bacterial strains (genotypes of various cloning hosts)
Chimiocompetent cells preparation (Not for DH10b)
DH10B chimiocompetent cells preparation
TSS competent cell preparation (Quick & dirty chimiocompetent cells)
Transformation of chimiocompetent cells
Electrocompetent cells preparation
Quick & dirty electrocompetent cells preparation
Transformation by electroporation
Smash and Grab Yeast plasmid prep
Yeast Transformation
Beta-Galactosidase Filter Lift Assay (yeast)